Strain Data Sheet

RBRC10293

Strain Information

Image
BRC No.RBRC10293
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Itpr2<tm2.1Kmik>
Former Common nameItpr2 floxed mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2018. ES cells derived from C57BL/6 were used.
Strain descriptionItpr2 (inositol 1,4,5-trisphosphate receptor 2) floxed mice. A neo gene fragment was removed by crossing with a Flp mouse line.
Colony maintenance
References
Abolished InsP3R2 function inhibits sweat secretion in both humans and mice.
Klar J, Hisatsune C, Baig S M, Tariq M, Johansson A C, Rasool M, Malik N A, Ameur A, Sugiura K, Feuk L, Mikoshiba K, Dahl N
J. Clin. Invest., 124(11) 4773-4780 (2014). 25329695

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
Itpr2
MGI:99418		inositol 1,4,5-triphosphate receptor 26Itpr2<tm2.1Kmik> targeted mutation 2.1, Katsuhiko Mikoshiba
  • isolated anhidrosis with normal sweat glands(MedGEN)
    • loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Itpr2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Mikoshiba (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Zhang M, Zhi N, Feng J, Liu Y, Zhang M, Liu D, Yuan J, Dong Y, Jiang S, Ge J, Wu S, Zhao X.
    ITPR2 Mediated Calcium Homeostasis in Oligodendrocytes is Essential for Myelination and Involved in Depressive-Like Behavior in Adolescent Mice.
    Adv Sci (Weinh) e2306498(2024) 38476116