Strain Data Sheet

RBRC10294

Strain Information

Image
BRC No.RBRC10294
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Ahcyl1<tm1.1Kmik>/1
Former Common nameIRBIT KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2009. TT2 ES cells were used and this line was backcrossed to C57BL/6 for 15 generations. C57BL/6 congenic line.
Strain descriptionAhcyl1 (S-adenosylhomocysteine hydrolase-like 1) floxed mice. FRT-flanked Neo gene is placed at intron 1 of the Ahcyl1 gene. A pair of loxP sites flank the FRT-flanked Neo and exon 2 of the Ahcyl1 gene. The neo and exon 2 were removed by crossing with a Cre line. TT2 ES cells were used and then this line was backcrossed to C57BL/6 for 15 generations. C57BL/6 congenic line. Homozygous mutants tend to be small in their body size. Two sublines are available as RBRC10294 and RBRC10295. This is subline #1.
Colony maintenance
References
IRBIT suppresses IP3 receptor activity by competing with IP3 for the common binding site on the IP3 receptor.
Ando H, Mizutani A, Kiefer H, Tsuzurugi D, Michikawa T, Mikoshiba K
Mol Cell. 22(6):795-806 (2006). 16793548

IRBIT, an inositol 1,4,5-trisphosphate receptor-binding protein, specifically binds to and activates pancreas-type Na+/HCO3- cotransporter 1 (pNBC1).
Shirakabe K, Priori G, Yamada H, Ando H, Horita S, Fujita T, Fujimoto I, Mizutani A, Seki G, Mikoshiba K
Proc. Natl. Acad. Sci. USA, 103(25):9542-9547 (2006). 16769890

IRBIT regulates CaMKIIalpha activity and contributes to catecholamine homeostasis through tyrosine hydroxylase phosphorylation.
Kawaai K, Mizutani A, Shoji H, Ogawa N, Ebisui E, Kuroda Y, Wakana S, Miyakawa T, Hisatsune C, Mikoshiba K
Proc. Natl. Acad. Sci. USA, 112(17):5515-5520 (2015). 25922519

Health Report

Examination Date / Room / Rack2025/01/07Room:3-ARack:HSentinel mouse program
2024/10/07Room:3-ARack:HSentinel mouse program
2024/04/08Room:3-ARack:HSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ahcyl1
MGI:2385184		S-adenosylhomocysteine hydrolase-like 13Ahcyl1<tm1.1Kmik> targeted mutation 1.1, Katsuhiko Mikoshiba
loxP phage P1 loxP3loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP site, Mouse Ahcyl1 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Mikoshiba (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data