Strain Data Sheet

RBRC10299

Strain Information

Image
BRC No.RBRC10299
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Tmem240<*>Y163*)908Kmik
Former Common nameTMEM240 Y163* Tg mice line 901, 908, 935,929
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Chihiro Hisatsune and Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2018. C57BL6/J genetic background.
Strain descriptionBAC transgenic mice harboring a mutated form of Tmem240(transmembrane protein 240). Amino acid sequence after Tyr at 163 aa was replaced with Myc-His tag in BAC clone (R23-261I9). Four transgenic lines are available (RBRC10298, 10299, 10300, 10301).
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Amp Ampicillin resistance gene (E. coli)UNAmp
Frt yeast FRT (flippase recombination target) siteUNFrt
Frt yeast FRT (flippase recombination target) siteUNFrt
His His tag (synthetic)UNHis
Tmem240 transmembrane protein 240UNTmem240<Tmem240<*>Y163*> transgene insertion 908, Katsuhiko Mikoshiba
myc-tag myc-epitope tag (human)UNmyc-tag

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal gait(MP:0001406)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse BAC clone (R23-261I9), human Myc-tag, Synthetic His-tag, Bovine BGH polyA, human ACTB polyA, yeast FRT (flippase recombination target) sites, E. coli Ampicillin resistance gene
    Research applicationFLP/frt system
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorKatsuhiko Mikoshiba (RIKEN)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data