Strain Information | |
---|---|
Image | |
BRC No. | RBRC10305 |
Type | CRISPR/Cas9![]() |
Species | Mus musculus |
Strain name | B6;B6D2-Lrit2<em1Tfur> |
Former Common name | Lrit2 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJmsSlc |
Appearance | |
Strain development | Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University in 2015. B6D2HF1-derived fertilized eggs were used and mice were crossed with C57BL/6J. Mixed genetic background. |
Strain description | Lrit2(leucine-rich repeat, immunoglobulin-like and transmembrane domains 2)mutant mice generated by the CRISPR/Cas9. One bp insertion at exon 2, resulting in conversion of Asn51 to stop codon. |
Colony maintenance | |
References | Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity. Ueno A, Omori Y, Sugita Y, Watanabe S, Chaya T, Kozuka T, Kon T, Yoshida S, Matsushita K, Kuwahara R, Kajimura N, Okada Y, Furukawa T Cell Reports, 22, 3548-3561 (2018). 29590622 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |||||||
---|---|---|---|---|---|---|---|
Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Lrit2 MGI:2444885 | leucine-rich repeat, immunoglobulin-like and transmembrane domains 2 | 14 | Lrit2<em1Tfur> | endonuclease-mediated mutation 1, Takahisa Furukawa |
Phenotype | |
---|---|
Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
---|---|
Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin] |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Reports, 22, 3548-3561 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University. |
Depositor | Takahisa Furukawa (Osaka University) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
|
BRC mice in Publications |
---|
No Data |