Strain Data Sheet

RBRC10315

Strain Information

Image
BRC No.RBRC10315
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6.B6D2-Bdh1<em1Fkao>
Former Common name
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJmsSlc
Appearance
Strain developmentDeveloped by Masatake Osawa in 2016 and deposited by Toshiyuki FUkao, Gifu University. This strain was generated by the Genome editing using fertilized eggs derived from B6D2, and then backcrossed to C57BL/6J for 5 generations.
Strain descriptionBdh1 (3-hydroxybutyrate dehydrogenase, type 1) mutant mice. 1 and 6 bp deletion at exon containing translation initiation codon. Bdh1<em1Fkao>: 1 and 6 bp deletionsCATGAGGGTGTACCTCGAGctgggcctagttggttaacttgaataacttgaagaagaaaatggctgttcttgactaaaactcccaagtggaaggcgctgagcatgcgttttgtgtttcagagccaccagaaggaagcagagcgtcttacaacttggtgccacgatgctagctgcccgtctctccagacccctgtcacagctcccaggaaaagccctaagtgtccgtgacagagaaaatgggacaaggtaagcaaccaaagggagggttggccttgaagaccaccctaaacatcttcactacctgccagacctcagtgctcaggagacaggacttcccgtggCTCACGGGGCAAATAGGAGT
Colony maintenance
References
Deficiency of 3-hydroxybutyrate dehydrogenase (BDH1) in mice causes low ketone body levels and fatty liver during fasting.
Otsuka H, Kimura T, Ago Y, Nakama M, Aoyama Y, Abdelkreem E, Matsumoto H, Ohnishi H, Sasai H, Osawa M, Yamaguchi S, Mitchell G A, Fukao T
J. Inherit. Metab. Dis., 43, 960-968 (2020). 32279332

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bdh1
MGI:1919161		3-hydroxybutyrate dehydrogenase, type 116Bdh1<em1Fkao> endonuclease-mediated mutation 1, Toshiyuki Fukao

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • increased circulating ketone body level(MP:0002575)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Inherit. Metab. Dis. 43, 960-968 (2020), doi:10.1002/jimd.12243
    DepositorToshiyuki Fukao (Gifu University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data