Strain Data Sheet

RBRC10317

Strain Information

Image
BRC No.RBRC10317
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Epc1<tm1Hko>
Former Common nameEpc1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6NCrl
Appearance
Strain developmentDeveloped by Haruhiko Koseki and Kyo-ichi Isono, RIKEN, in 2014. R1((129X1/SvJ x 129S1/Sv)F1) ES cells were used. This strain was backcrossed to C57BL/6J twice.
Strain descriptionEpc1 (enhancer of polycomb homolog 1) knockout mice.
Colony maintenance
References
EPC1/TIP60-Mediated Histone Acetylation Facilitates Spermiogenesis in Mice.
Dong Y, Isono K I, Ohbo K, Endo T A, Ohara O, Maekawa M, Toyama Y, Ito C, Toshimori K, Helin K, Ogonuki N, Inoue K, Ogura A, Yamagata K, Kitabayashi I, Koseki H
Mol. Cell Biol., 37(19) (2017). 28694333

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Epc1
MGI:1278322		enhancer of polycomb homolog 118Epc1<tm1Hko>
MGI:6153935		targeted mutation 1, Haruhiko Koseki
loxP phage P1 loxP18loxP
loxP phage P1 loxP18loxP
neo neomycin resistance gene (E. coli)18 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal X-Y chromosome synapsis during male meiosis(MP:0011751)

  • abnormal seminiferous tubule morphology(MP:0002216)

  • abnormal spermatocyte morphology(MP:0006379)

  • asthenozoospermia(MP:0002675)

  • decreased testis weight(MP:0004852)
    • more 2 phenotypes
  • postnatal growth retardation(MP:0001732)

  • reduced fertility(MP:0001921)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, mouse PGK promoter, E.coli neo, PGK pA, mouse Epc1 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data