Strain Data Sheet

RBRC10360

Strain Information

Image
BRC No.RBRC10360
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Il22ra2<tm1.1Ksat>
Former Common nameIl22ra2-/- mice
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuaki Sato, Miyazaki University in 2018. JN/2 ES cells derived from B6 were used, and then mice were backcrossed to C57BL/6J over 12 generations. C57BL/6 congenic strain. Stop cassette was knocked in at Exon 1 of the Il22ra2 gene.
Strain descriptionIl22ra2(interleukin 22 receptor, alpha 2) knockout mice.
Colony maintenance
References
Pivotal Role of IL-22 Binding Protein in the Epithelial Autoregulation of Interleukin-22 Signaling in the Control of Skin Inflammation.
Fukaya T, Fukui T, Uto T, Takagi H, Nasu J, Miyanaga N, Arimura K, Nakamura T, Koseki H, Choijookhuu N, Hishikawa Y, Sato K
Front. Immunol., 9, 1418 (2018). 29977242

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)10EGFP
HBEGF heparin-binding EGF-like growth factor (human)10HBEGF
Il22ra2
MGI:2665114		interleukin 22 receptor, alpha 210Il22ra2<tm1.1Ksat> targeted mutation 1.1, Katsuaki Sato
loxP phage P1 loxP10loxP
herpes simplex virus thymidine kinase (HSV TK) polyA10

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • increased susceptibility to chemically induced skin inflammation(MP:0012572)

  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNASTOP cassette (human HBEGF cDNA, jellyfish EGFP cDNA, herpes simplex virus thymidine kinase (HSV TK) poly A), Phage P1 loxP site, mouse Il22ra2 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Immunol., 9, 1418 (2018). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Dr. Katsuaki Sato) using the Approval Form. The Recipient acknowledges that RIKEN (and/or its original provider) reserves title to the Material. The Recipient will not transfer the Material to any scientists. The Recipient will not publish or present Research result without obtaining prior permission from the DEVELOPER (Dr. Katsuaki Sato). The Recipient will send Dr. Katsuaki Sato any abstract of submission, oral or written presentation, and/or copy of draft of paper or research article which is resulted from the Research at least 30 days prior to the submission. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEVELOPER (Dr. Katsuaki Sato).
    DepositorKatsuaki Sato (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data