Strain Data Sheet

RBRC10381

Strain Information

Image
BRC No.RBRC10381
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Arf6<tm1Yaka>
Former Common nameArf-6
H-2 Haplotype
ES Cell lineE14K [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Teruhiko Suzuki, University of Tsukuba in 2004. A neomycin resistance gene replaced a genomic region containing the translation initiation codon of the Arf6 gene. E14K ES cells dereived from 129P2/OlaHsd was used, and then mice were backcrossed to C57BL/6J over 10 generations.
Strain descriptionArf6 knockout mice. Homozygous mutants are embryonically lethal due to impaired liver development.
Colony maintenance
References
Crucial role of the small GTPase ARF6 in hepatic cord formation during liver development.
Suzuki T, Kanai Y, Hara T, Sasaki J, Sasaki T, Kohara M, Maehama T, Taya C, Shitara H, Yonekawa H, Frohman M A, Yokozeki T, Kanaho Y
Mol. Cell Biol., 26(16), 6149-56 (2006). 16880525

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Arf6
MGI:99435		ADP-ribosylation factor 612Arf6<tm1Yaka>
MGI:3662650		targeted mutation 1, Yasunori Kanaho
neo neomycin resistance gene (E. coli)12 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal hepatic cord morphology(MP:0012246)

  • abnormal liver development(MP:0000596)

  • abnormal liver morphology(MP:0000598)

  • anemia(MP:0001577)

  • decreased liver weight(MP:0003402)
    • more 6 phenotypes
  • hemorrhage(MP:0001914)

  • hydrops fetalis(MP:0002192)

  • lethality throughout fetal growth and development, incomplete penetrance(MP:0011109)

  • liver hypoplasia(MP:0000600)

  • pale liver(MP:0000603)

  • perinatal lethality, incomplete penetrance(MP:0011090)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK poly(A) signal, mouse Arf6 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Biol., 26(16), 6149-56. (2006).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorYasunori Kanaho (University of Tsukuba)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data