Strain Data Sheet

RBRC10382

Strain Information

Image
BRC No.RBRC10382
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Arf6<tm2.1Yaka>
Former Common nameArf6-flox
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Tunaki Hongu, University of Tsukuba in 2008. TT2 ES cells dereived from (C57BL/6NCrlj x CBA/JNCrlj)F1 was used, and then mice were backcrossed to C57BL/6J for more than 10 generations.
Strain descriptionArf6 floxed mice. A pair of loxP sites flank Exon 1 and 2 of the Arf6 gene. Homozygous floxed mice show no gross abnormality.
Colony maintenance
References
Trans-regulation of oligodendrocyte myelination by neurons through small GTPase Arf6-regulated secretion of fibroblast growth factor-2.
Akiyama M, Hasegawa H, Hongu T, Frohman M A, Harada A, Sakagami H, Kanaho Y
Nat. Commun., 5, 4744 (2014). 25144208

Arf6 regulates tumour angiogenesis and growth through HGF-induced endothelial beta1 integrin recycling.
Hongu T, Funakoshi Y, Fukuhara S, Suzuki T, Sakimoto S, Takakura N, Ema M, Takahashi S, Itoh S, Kato M, Hasegawa H, Mochizuki N, Kanaho Y
Nat. Commun., 6, 7925 (2015). 26239146

The small G protein Arf6 expressed in keratinocytes by HGF stimulation is a regulator for skin wound healing.
Miura Y, Ngo Thai Bich V, Furuya M, Hasegawa H, Takahashi S, Katagiri N, Hongu T, Funakoshi Y, Ohbayashi N, Kanaho Y
Sci. Rep., 7, 46649 (2017). 28429746

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Arf6
MGI:99435		ADP-ribosylation factor 612Arf6<tm2.1Yaka>
MGI:5698618		targeted mutation 2.1, Yasunori Kanaho
Frt yeast FRT (flippase recombination target) site12Frt
loxP phage P1 loxP12loxP
loxP phage P1 loxP12loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, yeast(S.crevisiae) FRT (flippase recombination target) site, mouse Arf6 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 5, 4744 (2014). Nat. Commun., 6, 7925 (2015). Sci. Rep., 7, 46649 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorYasunori Kanaho (University of Tsukuba)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data