Strain Data Sheet

RBRC10383

Strain Information

Image
BRC No.RBRC10383
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Pip5k1a<tm1Tba>
Former Common name
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Yasunori Kanaho and Takamitsu Unoki, University of Tsukuba in 2005. E14 ES cells derived from 129P2/OlaHsd was used, and then mice were backcrossed to C57BL/6J over 10 generations.
Strain description)Pip5k1a (phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha) knockout mice. A floxed neo fragment replaced Exon 5 of the Pip5k1a gene. Homozygous males are infertile.
Colony maintenance
References
Phosphatidylinositol 4-phosphate 5-kinase is indispensable for mouse spermatogenesis.
Hasegawa H, Noguchi J, Yamashita M, Okada R, Sugimoto R, Furuya M, Unoki T, Funakoshi Y, Baba T, Kanaho Y
Biol. Reprod., 86(5) 136, 1-12 (2012). 22321832

Regulation of HGF-induced hepatocyte proliferation by the small GTPase Arf6 through the PIP2-producing enzyme PIP5K1A.
Tsai M T, Katagiri N, Ohbayashi N, Iwasaki K, Ohkohchi N, Ding S T, Kanaho Y, Funakoshi Y
Sci. Rep., 7(1) 9438 (2017). 28842595

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pip5k1a
MGI:107929		phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha3Pip5k1a<tm1Tba> targeted mutation 1, Tadashi Baba
loxP phage P1 loxP3loxP
loxP phage P1 loxP3loxP
neo neomycin resistance gene (E. coli)3 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal mast cell physiology(MP:0002423)

  • abnormal reproductive system morphology(MP:0002160)

  • abnormal reproductive system physiology(MP:0001919)

  • abnormal sperm midpiece morphology(MP:0009831)

  • abnormal spermiation(MP:0004182)
    • more 12 phenotypes
  • asthenozoospermia(MP:0002675)

  • coiled sperm flagellum(MP:0009238)

  • decreased fertilization frequency(MP:0009647)

  • decreased testis weight(MP:0004852)

  • detached sperm flagellum(MP:0008893)

  • hematopoietic system phenotype(MP:0005397)

  • impaired fertilization(MP:0000242)

  • increased susceptibility to type I hypersensitivity reaction(MP:0005596)

  • lethality, incomplete penetrance(MP:0010831)

  • male infertility(MP:0001925)

  • oligozoospermia(MP:0002687)

  • reduced male fertility(MP:0001922)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Pip5k1a genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 86(5) 136, 1-12 (2012). Sci. Rep., 7(1) 9438 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
    DepositorYasunori Kanaho (University of Tsukuba)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data