Strain Data Sheet

RBRC10389

Strain Information

Image
BRC No.RBRC10389
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameICR.Cg-Kiss1<tm1.1Nurep>
Former Common nameKiss1-floxed (#96)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainCrlj:CD1(ICR)
Appearance
Strain developmentDeposited by Hiroko Tsukamura, Nagoya University. TT2 (CBAﾗC57BL/6) ES cells were used and then mice were crossed with ICR. Mixed genetic background. A neo fragment was deleted from this allele.
Strain descriptionKiss1 floxed mice. A pair of loxP sites flank Exon 3 of the Kiss1 gene.
Colony maintenance
References
Inducible Kiss1 knockdown in the hypothalamic arcuate nucleus suppressed pulsatile secretion of luteinizing hormone in male mice.
Minabe S, Nakamura S, Fukushima E, Sato M, Ikegami K, Goto T, Sanbo M, Hirabayashi M, Tomikawa J, Imamura T, Inoue N, Uenoyama Y, Tsukamura H, Maeda K I, Matsuda F
J. Reprod. Dev., 66(4):369-375 (2020). 32336702

Conditional kisspeptin neuron-specific Kiss1 knockout with newly generated Kiss1-floxed and Kiss1-Cre mice replicates a hypogonadal phenotype of global Kiss1 knockout mice.
Ikegami K, Goto T, Nakamura S, Watanabe Y, Sugimoto A, Majarune S, Horihata K, Nagae M, Tomikawa J, Imamura T, Sanbo M, Hirabayashi M, Inoue N, Maeda K I, Tsukamura H, Uenoyama Y
J. Reprod. Dev., 66(4):359-367 (2020). 32307336

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Kiss1
MGI:2663985		KiSS-1 metastasis-suppressor1Kiss1<tm1.1Nurep> targeted mutation 1.1, Lab. of Reproductive Science, Nagoya University
  • hypogonadotropic hypogonadism 13 with or without anosmia(DisGeNET, MedGEN)
    • loxP phage P1 loxP1loxP
    loxP phage P1 loxP1loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP sites, mouse Kiss1 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Reprod. Dev., 66(4):359-367 (2020). J. Reprod. Dev., 66(4):369-375 (2020). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Yoshihisa Uenoyama using the Approval Form. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of its use with the DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorHiroko Tsukamura (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data