Strain Data Sheet

RBRC10392

Strain Information

Image
BRC No.RBRC10392
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ebf3<tm1(cre/ERT2)Tng>
Former Common nameEbf3-CreERT2
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Masanari Seike, Osaka University in 2016. KY1.1(129/B6) ES cells were used, and then mice were backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic strain.
Strain descriptionEbf3-CreERT knock-in mice. These mice have a tamoxifen-inducible cre (CreERT2) at the Ebf3 locus. IRES-CreERT2 and FRT-PGKpro-neo-pA-FRT cassettes were inserted into the 3' UTR of exon 16 of the Ebf3 locus.
Colony maintenance
References
Stem cell niche-specific Ebf3 maintains the bone marrow cavity.
Seike M, Omatsu Y, Watanabe H, Kondoh G, Nagasawa T
Genes Dev., 32, 359-372 (2018). 29563184

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)7ERT2
Ebf3
MGI:894289		early B cell factor 37Ebf3<tm1(cre/ERT2)Tng> targeted mutation 1, Takashi Nagasawa
  • hypotonia, ataxia, and delayed development syndrome(MedGEN)
    • Frt yeast FRT (flippase recombination target) site7Frt
    Frt yeast FRT (flippase recombination target) site7Frt
    Ires internal ribosomal entry site (EMCV)7
    cre Phage P1 Cre recombinase7cre
    neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus Internal ribosomal entry site, Bacteriophage P1 Cre, Human ERT2 cDNA, Yeast Frt sites, Mouse PGK promoter, E. coli Neomycin resistant gene, Mouse PolyA signal, mouse Ebf3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takashi Nagasawa) and enter into a Material Transfer Agreement with Osaka University. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorTakashi Nagasawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Cre-ERT(2): NOTICE TO RECIPIENT
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Co-creation Bureau, Osaka University HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data