Strain Data Sheet
RBRC10444
Strain Information | |
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| Image | |
| BRC No. | RBRC10444 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;Cg-Gt(ROSA)26Sor<tm1(CAG-Cerulean/Arl13b,-Venus/GMNN,-Cherry/CDT1)Rmort> |
| Former Common name | R26Arl13bCerulean-Fucci2aR |
| H-2 Haplotype | |
| ES Cell line | E14 [129P2/OlaHsd] |
| Background strain | |
| Appearance | |
| Strain development | Fucci2 consists of the cell cycle phase specific biosensors mCherry-Cdt1(30/120) and mVenus-hGem(1/110) fused with the Thosea asigna virus 2A peptide sequence in a single bicistronic transgene (Mort et al., 2014).Arl13bCerulean consists of the full mouse Arl13b cDNA (OTTMUST00000058920.GRCm38) and mCerulean. Arl13bCerulean-Fucci2a couples Arl13bCerulean to Fucci2a using the porcine teschovirus-1 2A (P2A) peptide on the N-terminus of Fucci2a to create a tricistronic construct. The CAG promoter incorporates the cytomegalovirus (CMV) early enhancer; the promoter, first exon and intron of the chick β-actin gene; and the beta-globin splice acceptor (Miyazaki et al., 1989). |
| Strain description | Insertion by homologous combination in MESC. Targeting of CAG-floxNeo-Arl13bCerulean-Fucci2a inducible transgene to the mouse Rosa26 locus. No phenotype observed. Normal reproductive performance. |
| Colony maintenance | |
| References | Dev. Cell, 47, 509-523 (2018). 30458140 |
Health Report | |
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| Examination Date / Room / Rack | 2024/01/22Room:3-CRack:J 2023/10/23Room:3-CRack:J 2023/07/24Room:3-CRack:J 2023/04/24Room:3-CRack:J |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-Cerulean/Arl13b,-Venus/GMNN,-Cherry/CDT1)Rmort>targeted mutation 1 Richard Mort Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Arl13bADP-ribosylation factor-like 13B6Arl13b Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter CDT1human CDT1 cDNA6CDT1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GMNNgeminin (human)6GMNN Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter P2Aporcine teschovirus-1 2A peptide6P2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter T2AThosea asigna virus 2A peptide (insect)6T2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)6Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCeruleanCyan Fluorescent protein (Aequorea victoria)6mCeruleanCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal6 |
Ordering Information | |
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| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 phage loxP sites, E. coli neo, SV40 polyA, human CDT1 cDNA, Discosoma sp mCherry cDNA, T2A(Thosea asigna virus 2A peptide) , Jellyfish variant of enhanced yellow fluorescent protein (mVenus) cDNA, human GMNN cDNA, mouse Rosa26 genomic DNA, mouse Arl13b cDNA, P2A (porcine teschovirus-1 2A peptide), Aequorea victoria mCerulean cDNA, Bovine Growth hormone polyA |
| Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell, 47, 509-523 (2018).The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr Richard Mort of Lancaster University is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
| Depositor | Ian Jackson (University of Edinburgh) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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