Strain Data Sheet
RBRC10582
Strain Information | |
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| Image | |
| BRC No. | RBRC10582 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Adrm1<tm1Smta> |
| Former Common name | Rpn13f/fNeo |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Jun Hamazaki, The University of Tokyo in 2009. RENKA ES cells derived from C57BL/6N were used. Mice were further backcrossed to C57BL/6N for more than 6 generations. |
| Strain description | Adrm1 (adhesion regulating molecule 1, Rpn13 Rpn13) mutant mice. An FRT-flanked neomycin resistance cassette was inserted upstream of exon 3. This fragment and exon 4 were flanked by a pair of loxP. |
| Colony maintenance | |
| References | Redundant Roles of Rpn10 and Rpn13 in Recognition of Ubiquitinated Proteins and Cellular Homeostasis. Hamazaki J, Hirayama S, Murata S PLoS Genet., 11(7): e1005401 (2015). 26222436 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Adrm1 MGI:1929289 | adhesion regulating molecule 1 26S proteasome ubiquitin receptor | 2 | Adrm1<tm1Smta> MGI:5796186 | targeted mutation 1, Shigeo Murata | |||
| Frt | yeast FRT (flippase recombination target) site | 2 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 2 | Frt | ||||
| loxP | phage P1 loxP | 2 | loxP | ||||
| loxP | phage P1 loxP | 2 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 2 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Phage P1 1oxP sites, Yeast FRT (flippase recombination target) sites, Mouse phosphoglycerate kinase promoter (PGK promoter), Neomycin resistance gene (E.coli), Pgk polyA, mouse Adrm1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Hamazaki J, Hirayama S, Murata S. (2015) Redundant roles of Rpn10 and Rpnl3 in recognition of ubiquitinated proteins and cellular homeostasis . PLoS Genet.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Shigeo Murata (The University of Tokyo) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |