Strain Data Sheet
RBRC10587
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10587 |
| Type | CRISPR/Cas9 |
| Species | Mus musculus |
| Strain name | C57BL/6-Pnldc1<em1Nkn> |
| Former Common name | Pnldcl_del-1lnt-Exon3, Pnldc1-KO |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Toru Nakano, Graduate School of Frontier Biosciences, Osaka University. C57BL/6 genetic background. |
| Strain description | Pnldc1 (poly(A)-specific ribonuclease (PARN)-like domain containing 1) mutant mice. 11 bp deletion at Exon 3 generated with the Crispr/Cas9 technique and fertilized eggs derived from C57BL/6. Homozygous males are infertile, whereas homozygous females are fertile. |
| Colony maintenance | |
| References | PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. Nishimura T, Nagamori I, Nakatani T, Izumi N, Tomari Y, Kuramochi-Miyagawa S, Nakano T EMBO Rep., 19(3):e44957 (2018). 29444933 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Pnldc1 MGI:2685159 | poly(A)-specific ribonuclease (PARN)-like domain containing 1 | 17 | Pnldc1<em1Nkn> MGI:6159916 | endonuclease-mediated mutation 1, Toru Nakano | |||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | マウス Pnldc1 |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO Rep., 19(3) pii: e44957 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
| Depositor | Toru Nakano (Osaka University) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |