Strain Data Sheet
RBRC10590
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10590 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6;B6D2-Tg(Piwil2-Flag/NLS/Piwil4)#10Nkn |
| Former Common name | Tg-MiliP-FN-Miwi2 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Toru Nakano, Graduate School of Frontier Biosciences, Osaka University. A DNA construct was injected to fertilized eggs derived from BDF1, and then mice were backcrossed to C57BL/6. Mixed genetic background. |
| Strain description | Transgenic mice (Line 10) expressing Flag-NLS-MIWI2 under the control of mouse mili promoter. |
| Colony maintenance | |
| References | MIWI2 as an Effector of DNA Methylation and Gene Silencing in Embryonic Male Germ Cells. Kojima-Kita K, Kuramochi-Miyagawa S, Nagamori I, Ogonuki N, Ogura A, Hasuwa H, Akazawa T, Inoue N, Nakano T Cell Rep., 16(11):2819-2828 (2016). 27626653 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| FLAG | FLAG tag (synthetic) | UN | FLAG | mouse mili promoter | |||
| Piwil4 | piwi-like RNA-mediated gene silencing 4 | UN | Piwil4 | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | UN | nls | ||||
| SV40 polyA | UN | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Mouse Mili promoter, FLAG tag, SV40 NLS (nuclear localization signal), Mouse MIWI2 genomic DNA, SV40 polyA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep. 2016 Sep 13;16(11):2819-2828. do1: 10.1016/j.celrep.2016.08.027. PMID: 27626653In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. |
| Depositor | Toru Nakano (Osaka University) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |