Strain Data Sheet

RBRC10599

Strain Information

Image
BRC No.RBRC10599
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc6a4-GAP/GFP)1Ito
Former Common name5HTT-GAP43-EGFP, TCA-GFP, Sert-GAP43-EGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J. C57BL/6 genetic background.
Strain descriptionA BAC transgenic mouse in which GAP/GFP is under the control of the Slc6a4 (solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; 5Htt) gene promoter.
Colony maintenance
ReferencesNeuron, 82(2) 365-79 (2014). 24685175

Health Report

Examination Date / Room / Rack2024/03/11Room:3-5Rack:C
2023/12/11Room:3-5Rack:C
2023/09/11Room:3-5Rack:C
2023/06/13Room:3-5Rack:C
2023/05/22Room:3-5Rack:C
2023/03/13Room:3-5Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GAP43growth associated protein 43UNGAP43

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40UN

Ordering Information

Donor DNAmouse serotonin transporter(5HTTP) genomic DNA, GAP43 palmitoylation signal, jellyfish EGFP cDNA, SV40 polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mizuno et al. Neuron, 82(2) 365-79 (2014).
DepositorShigeyoshi Itohara (RIKEN CBS)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data