Strain Data Sheet
RBRC10599
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10599 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6J-Tg(Slc6a4-GAP/GFP)1Ito |
| Former Common name | 5HTT-GAP43-EGFP, TCA-GFP, Sert-GAP43-EGFP |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J. C57BL/6 genetic background. |
| Strain description | A BAC transgenic mouse in which GAP/GFP is under the control of the Slc6a4 (solute carrier family 6 (neurotransmitter transporter, serotonin), member 4; 5Htt) gene promoter. |
| Colony maintenance | |
| References | NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Mizuno H, Luo W, Tarusawa E, Saito Y M, Sato T, Yoshimura Y, Itohara S, Iwasato T Neuron, 82(2) 365-79 (2014). 24685175 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2024/12/09Room:3-5Rack:CSentinel mouse program 2024/09/09Room:3-5Rack:CSentinel mouse program 2024/06/10Room:3-5Rack:CSentinel mouse program 2024/03/11Room:3-5Rack:CSentinel mouse program 2023/12/11Room:3-5Rack:CSentinel mouse program 2023/09/11Room:3-5Rack:CSentinel mouse program 2023/06/13Room:3-5Rack:CSentinel mouse program 2023/05/22Room:3-5Rack:CAdditional tests |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| EGFP | Enhanced Green Fluorescent Protein (Aequorea victoria) | UN | EGFP | ||||
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| GAP43 | growth associated protein 43 | UN | GAP43 | ||||
| SV40 | UN | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | mouse serotonin transporter(5HTTP) genomic DNA, GAP43 palmitoylation signal, jellyfish EGFP cDNA, SV40 polyA, yeast FRT (flippase recombination target) site, Phage P1 loxP site derived from pBACe3.6 vector |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron, 82(2) 365-79 (2014). |
| Depositor | Shigeyoshi Itohara (RIKEN CBS) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |