Strain Data Sheet

RBRC10613

Strain Information

Image
BRC No.RBRC10613
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Usp15<tm1Tchi>
Former Common nameUSP15 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Tomoki Chiba, University of Tsukuba, in 2003. TT2 ES cell lines derived from (C57BL/6 x CBA)F1 were used, and then mice were backcrossed to C57BL/6J for more than 50 generations.
Strain descriptionUsp15 (ubiquitin specific peptidase 15) knock out mice.
Colony maintenance
References
USP15 Deubiquitinates TUT1 Associated with RNA Metabolism and Maintains Cerebellar Homeostasis.
Jaehyun Kim, Junnosuke Nakamura, Chiharu Hamada, Takumi Taketomi, Sarasa Yano, Tomomi Okajima, Shin-Ichi Kashiwabara, Tadashi Baba, Ban Sato, Tomoki Chiba, Fuminori Tsuruta
Mol. Cell Biol., 40(21):e00098-20 (2020). 32839293

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Usp15
MGI:101857		ubiquitin specific peptidase 1510Usp15<tm1Tchi> targeted mutation 1, Tomoki Chiba
neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal locomotor behavior(MP:0001392)

  • decreased body weight(MP:0001262)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, Polyomavirus SV40 polyA, mouse Usp15 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTomoki Chiba (University of Tsukuba)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data