Strain Data Sheet

RBRC10618

Strain Information

Image
BRC No.RBRC10618
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus molossinus
Strain nameMSM-Cdkn2a<em2Arak>/Yuwa
Former Common namepl9KOMSM-76
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Yuichi Wakabayashi, Chiba Cancer Center, in 2018. Developed by Kimi Araki, Kumamoto University using ES cells derived from MSM. CRISPR/Cas9 deleted exon 1 of the p16 (Cdkn2a).
Strain descriptionp19 Arf (Cdkn2a) knockout mice on MSM genetic background.
Colony maintenance
References
Identification of Stmm3 locus conferring resistance to late-stage chemically induced skin papillomas on mouse chromosome 4 by congenic mapping and allele-specific alteration analysis.
Saito M, Okumura K, Miura I, Wakana S, Kominami R, Wakabayashi Y
Exp. Anim., 63(3):339-48 (2014). 25077764

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cdkn2a
MGI:104738		cyclin dependent kinase inhibitor 2A4Cdkn2a<em2Arak> endonuclease-mediated mutation 2, Kimi Araki

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYuichi Wakabayashi (Chiba Cancer Center)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data