Strain Data Sheet

RBRC10621

Strain Information

Image
BRC No.RBRC10621
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6.Cg-Aprt<em1Chum>
Former Common name
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichiro Chuma and Ami Katanaya, Institute for Frontier Life and Medical Sciences, Kyoto University in 2016. V6.5 ES cells derived from (129☓C57BL/6) were used. The strain was backcrossed to C57BL/6J for 4 generations.
Strain descriptionAprt (adenine phosphoribosyl transferase) mutant mice. A 475bp genomic DNA including exon 2 and 4 was deleted.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aprtadenine phosphoribosyl transferase8Aprt<em1Chum>endonuclease-mediated mutation 1, Shinichiro Chuma

Ordering Information

Donor DNA
Research application
Specific Term and Conditions・ Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval form the
DEVELOPER (Dr. Shinichiro Chuma) using the Approval Form.
・ The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a non-for profit
organization for a research and teaching purpose.
DepositorShinichiro Chuma (Kyoto University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data