Strain Data Sheet

RBRC10628

Strain Information

Image
BRC No.RBRC10628
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tgfbr2<tm1(cre/Esr1<*>)Sleep>
Former Common nameTgfbr2-CreERT2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. ES cell line derived from C57BL/6J was used. C57BL/6J genetic background.
Strain descriptionKnockin mice in which Cre/ERT2 was inserted at the translation initiation codon of the Tgfbr2 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actb chicken Beta-actin polyA9Actb
Esr1* human Estrogen receptor9Esr1*
Frt yeast FRT (flippase recombination target) site9Frt
P2A porcine teschovirus-1 2A peptide9P2A
Tgfbr2
MGI:98729		transforming growth factor, beta receptor II9Tgfbr2<tm1(cre/Esr1<*>)Sleep> targeted mutation, Yu Hayashi
  • colorectal cancer, hereditary nonpolyposis, type 6(MedGEN)

  • esophageal cancer(MedGEN)

  • Loeys-Dietz syndrome 2(MedGEN)
    • more 1 Diseases
  • Loeys-Dietz syndrome(DisGeNET)
    • cre Phage P1 Cre recombinase9cre

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), Bacteriophage P1 Cre recombinase, Homo sapiens Estrogen receptor cDNA, chicken Beta-actin polyA, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli Neomycin resistance gene, mouse Tgfbr2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorYu Hayashi (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data