Strain Data Sheet

RBRC10633

Strain Information

Image
BRC No.RBRC10633
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Chat-cre)609Sleep
Former Common nameChat-Cre Tg609
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 609) expressing Cre under the control of Chat promoter.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/04/22Room:3-CRack:JSentinel mouse program
2024/01/22Room:3-CRack:JSentinel mouse program
2023/10/23Room:3-CRack:JSentinel mouse program
2023/07/24Room:3-CRack:JSentinel mouse program
2023/04/24Room:3-CRack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Actb chicken Beta-actin polyAUNActb
Frt yeast FRT (flippase recombination target) siteUNFrt
cre Phage P1 Cre recombinaseUNcre
loxP phage P1 loxPUNloxP
neo neomycin resistance gene (E. coli)UN
nls SV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAMus musculus Chat genomic DNA (BAC clone RP23-246B12), SV40 Nuclear Localization Signal(NLS), Bacteriophage P1 Cre, Chicken Beta-actin polyA, Saccharomyces cerevisiae FRT site, Phage P1 loxP site derived from pBACe3.6 vector, E. coli neomycin resistance gene
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYu Hayashi (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data