Strain Data Sheet

RBRC10637

Strain Information

Image
BRC No.RBRC10637
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Chat-neo,-tTA)838Sleep
Former Common nameChat-loxP-stop-loxP-tTA Tg838
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A BAC transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 838) expressing tTA under the control of Chat promoter and Cre recombinase.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2025/01/20Room:3-CRack:KSentinel mouse program
2024/10/21Room:3-CRack:KSentinel mouse program
2024/07/22Room:3-CRack:KSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
loxP phage P1 loxPUNloxP
loxP phage P1 loxPUNloxP
neo neomycin resistance gene (E. coli)UN
tTA tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTA
SV40 Intron-polyAUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAMus musculus Chat gonomic DNA, Bacteriophage P1 loxP sites, phage T7 gb2 promoter, Escherichia coli Neomycin resistance gene, Escherichia coli tTA, SV40 Intron-polyA
Research applicationCre/loxP system
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYu Hayashi (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data