Strain Data Sheet

RBRC10646

Strain Information

Image
BRC No.RBRC10646
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-Kcnj2/EGFP)20Sleep
Former Common nametetO-Kir2.1-EGFP Tg20
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yu Hayashi at University of Tsukuba and Shigeyoshi Itohara at RIKEN. A transgenic DNA construct was injected to C57BL/6J. C57BL/6J genetic background.
Strain descriptionTransgenic mice (line 20) expressing EGFP-fused Kir2.1 under the control of tetO promoter.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2023/10/10Room:3-BRack:H
2023/07/10Room:3-BRack:H
2023/04/10Room:3-BRack:H
2023/01/10Room:3-BRack:H
2022/10/11Room:3-BRack:H
2022/07/11Room:3-BRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kcnj2potassium inwardly-rectifying channel, subfamily J, member 2UNKcnj2E. coli tetO (tetracycline operator)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 Intron-polyAUN

Ordering Information

Donor DNACMV promoter, E. coli tetO (tetracycline operator), Mus musculus Kir2.1 cDNA, Aequorea victoria GFP cDNA, SV40 Intron-polyA
Research applicationFluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorYu Hayashi (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data