Strain Data Sheet
RBRC10708
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10708 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(CAG-flpe)32Ito |
| Former Common name | CAG-FLPe |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Hiroaki Kanki and Shigeyoshi Itohara, RIKEN Brain Science Institute in 2006. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6J background. |
| Strain description | Transgenic mice expressing Flpe under the control of CAG promoter (Line 32). |
| Colony maintenance | |
| References | High-efficiency CAG-FLPe deleter mice in C57BL/6J background. Kanki H, Suzuki H, Itohara S Exp. Anim., 55, 137-141 (2006). 16651697 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Ires | internal ribosomal entry site (EMCV) | UN | |||||
| flpe | yeast flippase recombinase | UN | flpe | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| nls | SV40 large T antigen nuclear localization signal (NLS) | UN | nls | ||||
| puro | puromycin acyltransferase (Streptomyces alboniger) | UN | puro | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | サイトメガロウイルス 初期遺伝子エンハンサー, ニワトリ アクチン遺伝子転写調節領域, 酵母 FLPeリコンビネースコーディング配列, ウサギ beta グロブリン転写調節領域(polyA配列), SV40 Nuclear Localization Signal(NLS), ウシ BGH転写終結配列, ECMV IRES, Streptomyces alboniger puromycin resistance gene |
| Research application | FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 55, 137-141 (2006). |
| Depositor | Shigeyoshi Itohara (RIKEN) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
|---|
| No Data |