Strain Data Sheet
RBRC10744
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10744 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6-Eno2<tm1(DTA)Hsak>/Ito |
| Former Common name | NSE-DTA |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shigeyoshi Itohara at RIKEN. |
| Strain description | Cre-dependent DTA expression cassettes was knocked in at the Eno2 locus. |
| Colony maintenance | |
| References | Innate versus learned odour processing in the mouse olfactory bulb. Kobayakawa K, Kobayakawa R, Matsumoto H, Oka Y, Imai T, Ikawa M, Okabe M, Ikeda T, Itohara S, Kikusui T, Mori K, Sakano H Nature, 450(7169) 503-508 (2007). 17989651Genetic dissection of medial habenula-interpeduncular nucleus pathway function in mice. Kobayashi Y, Sano Y, Vannoni E, Goto H, Suzuki H, Oba A, Kawasaki H, Kanba S, Lipp H P, Murphy N P, Wolfer D P, Itohara S Front. Behav. Neurosci., 7:17 (2013). 23487260 |
Health Report | |
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| Examination Date / Room / Rack | 2025/01/07Room:3-ARack:GSentinel mouse program 2024/10/07Room:3-ARack:GSentinel mouse program 2024/04/08Room:3-ARack:GSentinel mouse program 2024/01/09Room:3-ARack:GSentinel mouse program 2023/10/16Room:3-ARack:GAdditional tests 2023/10/10Room:3-ARack:GSentinel mouse program 2023/07/10Room:3-ARack:GSentinel mouse program 2023/04/10Room:3-ARack:GSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| DTA | diphtheria toxin A (DT-A) | 6 | DTA | ||||
| Eno2 MGI:95394 | enolase 2, gamma neuronal | 6 | Eno2<tm1(DTA)Hsak> MGI:3765961 | targeted mutation 1, Hitoshi Sakno | |||
| Ires | internal ribosomal entry site (EMCV) | 6 | |||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 6 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 loxP sites, mouse phosphoglycerate kinase promoter (Pgk promoter), E.coli neo resistance gene, C.diphtheriae diphtheria toxin A fagment, mouse Eno2 genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 7:17 (2013). |
| Depositor | Shigeyoshi Itohara (RIKEN) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |