Strain Data Sheet

RBRC10747

Strain Information

Image
BRC No.RBRC10747
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Rapgef3<tm1.2Ito> Rapgef4<tm1.1Ito>
Former Common nameCGIKO;CG2 KO
H-2 Haplotype
ES Cell lineMS12 [C57BL/6]
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara at RIKEN.
Strain descriptionDouble knockout for the genes, Rapgef3 and Rapgef4. Exon 1 and 2 or Exon 3 was deleted in Rapgef3 or Rapgef4 gene, respectively.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/12/09Room:3-6Rack:BSentinel mouse program
2024/09/09Room:3-6Rack:BSentinel mouse program
2024/06/10Room:3-6Rack:BSentinel mouse program
2024/03/11Room:3-6Rack:BSentinel mouse program
2023/12/11Room:3-6Rack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Rapgef3
MGI:2441741		Rap guanine nucleotide exchange factor (GEF) 315Rapgef3<tm1.2Ito> targeted mutation 1.2, Shigeyoshi Itohara
loxP phage P1 loxP15loxP
Rapgef4
MGI:1917723		Rap guanine nucleotide exchange factor (GEF) 42Rapgef4<tm1.1Ito> targeted mutation 1.1, Shigeyoshi Itohara
loxP phage P1 loxP2loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAphage P1 loxP site, mouse Rapgef3 genomic DNA, mouse Rapgef4 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data