Strain Information | |
---|---|
Image | |
BRC No. | RBRC10757 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Mab21l2<tm1Nao> |
Former Common name | C57BL/6(Mab21l2tm1Nao), Mab21l2tm1Nao |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | lethality throughout fetal growth and development, complete penetranceabnormal heart position or orientationabnormal retina morphologyabnormal ventral body wall morphology |
Strain description | This gene is similar to the MAB-21 cell fate-determining gene found in C. elegans. It is required for several aspects of embryonic development including normal development of the eye, heart and body wall. Mutation details: The entire gene coding region was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutants was confirmed by whole mount in situ hybridization analysis of E10.5 homozygous mutant embryos. |
Colony maintenance | |
References | Dev. Biol., 274(2):295-307 (2004). 15385160 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA3 |
Ordering Information | |
---|---|
Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Mab21l2 genomic DNA |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Naoki Takahashi (The University of Tokyo) |
Strain Status | Live mice Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |