Strain Data Sheet
RBRC10762
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10762 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.B6CB-Gsdmd<tm1.2Tshir>/Ms |
| Former Common name | Gsdmdflox/flox, Gsdmdfl/fl, Gsdmd cKO |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Toshihiko Shiroishi and Tomoaki Fujii, National Institute of Genetics in 2005. TT2 ES cells derived from (C57BL/6 x CBA)F1 were used. Mice were backcrossed to C57BL/6J for more than 10 generations. C57BL/6 congenic line. Frt-flanked Neo cassette was deleted by crossing with Flp mice. |
| Strain description | Floxed mice for Gsdmd. |
| Colony maintenance | |
| References | Gasdermin D (Gsdmd) is dispensable for mouse intestinal epithelium development. Fujii T, Tamura M, Tanaka S, Kato Y, Yamamoto H, Mizushina Y, Shiroishi T Genesis, 46, 418-423 (2008). 18693275Gasdermin D represses inflammation-induced colon cancer development by regulating apoptosis. Shigekazu Tanaka, Hajime Orita, Taro Kataoka, Masahiro Miyazaki, Harumi Saeki, Ryo Wada, Malcolm V Brock, Tetsu Fukunaga, Takanori Amano, Toshihiko Shiroishi Carcinogenesis, 44(4):341-349 (2023). 36753047 |
Health Report | |
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| Examination Date / Room / Rack | 2024/11/25Room:4-CRack:ESentinel mouse program 2024/08/26Room:4-CRack:ESentinel mouse program 2024/05/27Room:4-CRack:ESentinel mouse program 2024/02/26Room:4-CRack:ESentinel mouse program 2023/11/27Room:4-CRack:ESentinel mouse program 2023/08/28Room:4-CRack:ESentinel mouse program 2023/05/29Room:4-CRack:ESentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 15 | Frt | ||||
| Gsdmd MGI:1916396 | gasdermin D | 15 | Gsdmd<tm1.2Tshir> | targeted mutation 1.2, Toshihiko Shiroishi | |||
| loxP | phage P1 loxP | 15 | loxP | ||||
| loxP | phage P1 loxP | 15 | loxP | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | P1 bacteriophage loxP sites, S. cerevisiae Frt site, mouse Gsdmd genomic DNA |
| Research application | Cre/loxP system FLP/frt system Immunology and Inflammation Research |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 46, 418-423 (2008). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. (Contact info: Tsuyoshi Koide, Ph.D. <tkoide@nig.ac.jp>, Mouse Genomics Resource Laboratory) |
| Depositor | Toshihiko Shiroishi (National Institute of Genetics) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Mar 2020 |
BRC mice in Publications |
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Yoon SH, Kim CY, Lee E, Lee C, Lee KS, Lee J, Park H, Choi B, Hwang I, Kim J, Kim TG, Son J, Hyun YM, Hong S, Yu JW. Microglial NLRP3-gasdermin D activation impairs blood-brain barrier integrity through interleukin-1β-independent neutrophil chemotaxis upon peripheral inflammation in mice. Nat Commun 16(1) 699(2025) 39814731 |
Ma H, Jia H, Zou W, Ji F, Wang W, Zhao J, Yuan C, Jiao J. Gasdermin D Mediated Mitochondrial Metabolism Orchestrate Neurogenesis Through LDHA During Embryonic Development. Adv Sci (Weinh) 11(35) e2402285(2024) 39033542 |
Shim DW, Eo JC, Kim S, Hwang I, Nam B, Shin JE, Han SH, Yu JW. Deficiency of circadian clock gene Bmal1 exacerbates noncanonical inflammasome-mediated pyroptosis and lethality via Rev-erbα-C/EBPβ-SAA1 axis. Exp Mol Med 56(2) 370-382(2024) 38297162 |