Strain Information | |
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Image | |
BRC No. | RBRC10764 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Shh<tm1Tshir>/Ms |
Former Common name | Shhd2EGFP, Shh-d2EGFP KI |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Toshihiko Shiroishi and Takanori Amano, National Institute of Genetics in 2006 using ES cells derived from C57BL/6. C57BL/6 genetic background. |
Strain description | Degraded form of EGFP was knocked in at the Shh locus. |
Colony maintenance | |
References | Dev. Cell. l6(1):47-57 (2009). 19097946 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)5EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal5 |
Ordering Information | |
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Donor DNA | Jellyfish (A. victoria) EGFP cDNA, Mouse The degradation domain of ornithine decarboxylase cDNA, SV40 polyA signal, mouse PGK promoter, E. coli Neomycin resistance gene, mouse Shh genomic DNA |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Amano et al., Dev Cell. 2009 Jan;l6(1):47-57. |
Depositor | Toshihiko Shiroishi (National Institute of Genetics) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |