Strain Data Sheet

RBRC10764

Strain Information

Image
BRC No.RBRC10764
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Shh<tm1Tshir>/Ms
Former Common nameShhd2EGFP, Shh-d2EGFP KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Takanori Amano, National Institute of Genetics in 2006 using ES cells derived from C57BL/6. C57BL/6 genetic background.
Strain descriptionDegraded form of EGFP was knocked in at the Shh locus.
Colony maintenance
References
Chromosomal dynamics at the Shh locus: limb bud-specific differential regulation of competence and active transcription.
Amano T, Sagai T, Tanabe H, Mizushina Y, Nakazawa H, Shiroishi T
Dev. Cell. l6(1):47-57 (2009). 19097946

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)5EGFP
Shh
MGI:98297		sonic hedgehog5Shh<tm1Tshir>
MGI:3833929		targeted mutation 1, Toshihiko Shiroishi
  • holoprosencephaly 3(DisGeNET, MedGEN)

  • microphthalmia, isolated, with coloboma 5(MedGEN)

  • schizencephaly(MedGEN)
    • more 1 Diseases
  • solitary median maxillary central incisor syndrome(MedGEN)
    • neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)
    SV40 polyA signal5

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish (A. victoria) EGFP cDNA, Mouse The degradation domain of ornithine decarboxylase cDNA, SV40 polyA signal, mouse PGK promoter, E. coli Neomycin resistance gene, mouse Shh genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Amano et al., Dev Cell. 2009 Jan;l6(1):47-57.
    DepositorToshihiko Shiroishi (National Institute of Genetics)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data