Strain Data Sheet
RBRC10765
Strain Information | |
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| Image | |
| BRC No. | RBRC10765 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.129-Rr29<tm1.1Tshir>/Ms |
| Former Common name | MFCS1-/-, MFCS1 KO, ZRS KO |
| H-2 Haplotype | |
| ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Toshihiko Shiroishi and Tomoko Sagai, National Institute of Genetics in 2004 using R1 ES cells. C57BL/6 congenic line. |
| Strain description | Deletion mutant of the MFCS1 (Rr29) enhancer of Shh located with intron 5 of Lmbr1. |
| Colony maintenance | |
| References | Elimination of a long-range cis-regulatory module causes complete loss of limb-specific Shh expression and truncation of the mouse limb. Sagai T, Hosoya M, Mizushina Y, Tamura M, Shiroishi T Development, 132(4):797-803 (2005). 15677727 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| GH | Growth hormone polyA (Bovine) | 5 | GH | ||||
| Rr29 MGI:5499537 | regulatory region 29 | 5 | Rr29<tm1.1Tshir> MGI:6143996 | targeted mutation 1.1, Toshihiko Shiroishi | |||
| neo | neomycin resistance gene (E. coli) | 5 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | E.coli Neomycin resistant gene, mouse PGK promoter, bovine polyA signal |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2005 Feb;132(4):797-803. |
| Depositor | Toshihiko Shiroishi (National Institute of Genetics) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |