Strain Data Sheet

RBRC10766

Strain Information

Image
BRC No.RBRC10766
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Rr45<tm1.1Tshir>/Ms
Former Common nameMFCS4-/-, MFCS4 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Tomoko Sagai, National Institute of Genetics in 2007 using TT2 ES cells. C57BL/6 congenic line.
Strain descriptionDeletion mutant of the MFCS4 (Rr45) enhancer of Shh located within intron 8 of Rnf32.
Colony maintenance
ReferencesDevelopment, 136(10):1665-74 (2009). 19369396

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rr45regulatory region 455Rr45<tm1.1Tshir>targeted mutation 1.1, Toshihiko Shiroishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)5GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse PGK promoter, E.coli Neomycin resistant gene, bovine polyA signal
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development.(2009)136:1665-74.
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data