Strain Data Sheet

RBRC10766

Strain Information

Image
BRC No.RBRC10766
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Rr45<tm1.1Tshir>/Ms
Former Common nameMFCS4-/-, MFCS4 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Tomoko Sagai, National Institute of Genetics in 2007 using TT2 ES cells. C57BL/6 congenic line.
Strain descriptionDeletion mutant of the MFCS4 (Rr45) enhancer of Shh located within intron 8 of Rnf32.
Colony maintenance
References
A cluster of three long-range enhancers directs regional Shh expression in the epithelial linings.
Sagai T, Amano T, Tamura M, Mizushina Y, Sumiyama K, Shiroishi T
Development, 136(10):1665-74 (2009). 19369396

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)5GH
Rr45
MGI:6117281		regulatory region 455Rr45<tm1.1Tshir>
MGI:6117321		targeted mutation 1.1, Toshihiko Shiroishi
neo neomycin resistance gene (E. coli)5 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse PGK promoter, E.coli Neomycin resistant gene, bovine polyA signal
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development.(2009)136:1665-74.
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data