Strain Data Sheet

RBRC10771

Strain Information

Image
BRC No.RBRC10771
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Rr45<tm1.1Tshir> Rr(MRCS1)<tm1.1Tshir>/Ms
Former Common nameMRCS1-MFCS4 double knockout (DKO), delta MRCS1; delta MFCS4, MRCS1-/-; MFCS4-/-
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Tomoko Sagai, National Institute of Genetics in 2013 using TT2 ES cells. C57BL/6 congenic line.
Strain descriptionDeletion mutant of MRCS1 and MFCS4 enhancer of Shh gene.
Colony maintenance
References
A cluster of three long-range enhancers directs regional Shh expression in the epithelial linings.
Sagai T, Amano T, Tamura M, Mizushina Y, Sumiyama K, Shiroishi T
Development, 136(10):1665-74 (2009). 19369396

SHH signaling directed by two oral epithelium-specific enhancers controls tooth and oral development.
Sagai T, Amano T, Maeno A, Kiyonari H, Seo H, Cho S W, Shiroishi T
Sci. Rep., 7(1):13004 (2017). 29021530

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
9530036O11Rik
MGI:2444607		RIKEN cDNA 9530036O11Rik59530036O11Rik<tm1.1Tshir> targeted mutation 1.1, Toshihiko Shiroishi
Rr45
MGI:6117281		regulatory region 455Rr45<tm1.1Tshir>
MGI:6117321		targeted mutation 1.1, Toshihiko Shiroishi

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAmouse PGK promoter, E.coli Neomycin resistant gene, bovine poly A signal, P1 phage loxP
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. (2017) 7:13004. Development. (2009)136:1665-74.
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data