Strain Data Sheet

RBRC10791

Strain Information

Image
BRC No.RBRC10791
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Prox1<tm1.1Ykaw>
Former Common name
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Yoshiya Kawaguchi, Kyoto University in 2019. Bruce4 ES cells were used to generate this strain. Mice were crossed with C57BL/6J.
Strain descriptionFloxed Prox1 mice. A pair of loxP flanks Exon 2 of the Prox1 gene.
Colony maintenance
References
Liver-specific Prox1 inactivation causes hepatic injury and glucose intolerance in mice.
Goto T, Elbahrawy A, Furuyama K, Horiguchi M, Hosokawa S, Aoyama Y, Tsuboi K, Sakikubo M, Hirata K, Masui T, Kubo H, Sakai Y, Uemoto S, Kawaguchi Y
FEBS Lett., 591(4):624-635 (2017). 28129664

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site1Frt
Prox1
MGI:97772		prospero homeobox 11Prox1<tm1.1Ykaw>
MGI:6203069		targeted mutation 1.1, Yoshiya Kawaguchi
loxP phage P1 loxP1loxP
loxP phage P1 loxP1loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNABacteriophage P1 LoxP sites, yeast FRT (flippase recombination target) site, mouse Prox1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FEBS Lett., 591(4):624-635 (2017).
DepositorYoshiya Kawaguchi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Lin Q, Wu L, Chatla S, Chowdhury FA, Atale N, Joseph J, Du W.
Hematopoietic stem cell regeneration through paracrine regulation of the Wnt5a/Prox1 signaling axis.
J Clin Invest 132(12) (2022) 35703178