Strain Data Sheet

RBRC10798

Strain Information

Image
BRC No.RBRC10798
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Slc32a1-floxedSTOP-tdTomato)58Rkan
Former Common nameVGAT-stop-tdTomato line #58, VGAT-stop-tdTomato Chr.X, VsTB58
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ryosuke Kaneko and Yuchio Yanagawa, Gunma University in 2010. C57BL/6J genetic background. Transgene is integrated around the Hdx gene at Chromosome X.
Strain descriptionBAC transgenic mice (line 58) expressing tdTomato under the control of the Slc32a1 promoter and cre recombinase.
Colony maintenance
References
Inhibitory neuron-specific Cre-dependent red fluorescent labeling using VGAT BAC-based transgenic mouse lines with identified transgene integration sites.
Kaneko R, Takatsuru Y, Morita A, Amano I, Haijima A, Imayoshi I, Tamamaki N, Koibuchi N, Watanabe M, Yanagawa Y
J. Comp. Neurol., 526(3):373-396 (2018). 29063602

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteXFrt
HBB2 rabbit beta-globin polyAXHBB2
STOP human beta-globin polII terminatorXSTOP
loxP phage P1 loxPXloxP
loxP phage P1 loxPXloxP
tdTomato tandem dimeric Red Fluorescent Protein (Discosoma sp.)XtdTomato

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAマウス Slc32a1, ヒト beta-globin polII 転写終結シグナル, マウス GAD65 intron, バクテリオファージ loxP, 酵母 FRT, 珊瑚 tdTomato, ウサギ beta-globin polyA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp. Neurol., 526(3):373-396 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results form the use of the BIOLOGICAL RESOURCE.
DepositorRyosuke Kaneko (Gunma University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data