Strain Information | |
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Image | |
BRC No. | RBRC10799 |
Type | Transgene![]() |
Species | Mus musculus |
Strain name | C57BL/6-Tg(Slc32a1-tdTomato)54Rkan |
Former Common name | VGAT-tdTomato line #54 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Ryosuke Kaneko and Yuchio Yanagawa, Gunma University in 2010. C57BL/6J genetic background. Transgene is integrated around Gm12442 at Chromosome 4. |
Strain description | BAC transgenic mice (line 54) expressing tdTomato under the control of the Slc32a1 promoter. Stop cassette between Slc32a1 promoter and tdTomato was removed by crossing with Cre mice. |
Colony maintenance | |
References | Inhibitory neuron-specific Cre-dependent red fluorescent labeling using VGAT BAC-based transgenic mouse lines with identified transgene integration sites. Kaneko R, Takatsuru Y, Morita A, Amano I, Haijima A, Imayoshi I, Tamamaki N, Koibuchi N, Watanabe M, Yanagawa Y J. Comp. Neurol., 526(3):373-396 (2018). 29063602 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 4 | Frt | ||||
HBB2 | rabbit beta-globin polyA | 4 | HBB2 | ||||
loxP | phage P1 loxP | 4 | loxP | ||||
tdTomato | tandem dimeric Red Fluorescent Protein (Discosoma sp.) | 4 | tdTomato |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | mouse Slc32a1 promoter, mouse Gad2(GAD65) intron, phage P1 loxP site, yeast FRT (flippase recombination target) site, Discosoma sp.tdTomato cDNA, rabbit beta-globin polyA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp. Neurol., 526(3):373-396 (2018). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results form the use of the BIOLOGICAL RESOURCE. |
Depositor | Ryosuke Kaneko (Gunma University) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |