Strain Data Sheet

RBRC10843

Strain Information

Image
BRC No.RBRC10843
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rit1<tm1(A57G)Tumg>
Former Common nameRit1A57G Neo/+ (C57BL/6J background)
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2019. BRUCE-4 ES cells were used. C57BL/6 congenic line.
Strain descriptionKnock-in mutants in which a point mutation (A57G) was introduced at Exon 4 of the Rit1 gene. Floxed stop cassette and Frt-flnaked Neo cassette remain at intron 3.
Colony maintenance
References
New Noonan syndrome model mice with RIT1 mutation exhibit cardiac hypertrophy and susceptibility to beta-adrenergic stimulation-induced cardiac fibrosis.
Takahara S, Inoue S I, Miyagawa-Tomita S, Matsuura K, Nakashima Y, Niihori T, Matsubara Y, Saiki Y, Aoki Y
EBioMedicine, 42:43-53 (2019). 30898653

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site3Frt
Frt yeast FRT (flippase recombination target) site3Frt
Rit1
MGI:108053		Ras-like without CAAX 13Rit1<tm1(A57G)Tumg> targeted mutation 1, Yoko Aoki
  • Noonan syndrome 8(DisGeNET, MedGEN)

  • Noonan syndrome(DisGeNET)
    • SA Adenovirus SA(splice acceptor site)-stop3SA
    loxP phage P1 loxP3loxP
    loxP phage P1 loxP3loxP
    neo neomycin resistance gene3 mouse phosphoglycerate kinase promoter (PGK promoter)
    SV40 polyA signal3

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, Adenovirus SA(splice acceptor site)-stop, Polyomavirus SV40 polyA signal, Saccharomyces cerevisiae FRT sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, mouse Rit1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine, 42:43-53 (2019).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorYoko Aoki (Tohoku University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Nov 2022

    BRC mice in Publications

    No Data