Strain Data Sheet

RBRC10849

Strain Information

Image
BRC No.RBRC10849
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Tet2<tm1.1Hko>
Former Common nameTET2
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN in 2011. M1 ((129 x C57BL/6)F1) ES cells were used. This strain was backcrossed to C57BL/6J.
Strain descriptionTet2 gene floxed mice. Gene targeting for the Tet2 allele was done by flanking exon 9 with Cre-loxP sequences. Conditional deletion of Tet2 (exon 9) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The TET2 protein is a dioxygenase that catalyzes the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and further oxidized cytosine (5fC and 5caC), and plays a key role in active DNA demethylation. This conditional allele would be useful to study the roles of Tet2 in DNA de-methylation by combining with tissue specific Cre deleters.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2025/01/07Room:3-BRack:DSentinel mouse program
2024/10/07Room:3-BRack:DSentinel mouse program
2024/04/08Room:3-BRack:DSentinel mouse program
2024/01/09Room:3-BRack:DSentinel mouse program
2023/10/10Room:3-BRack:DSentinel mouse program
2023/07/10Room:3-BRack:DSentinel mouse program
2023/04/10Room:3-BRack:DSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site3Frt
Tet2
MGI:2443298		tet methylcytosine dioxygenase 23Tet2<tm1.1Hko> targeted mutation 1.1, Haruhiko Koseki
  • immunodeficiency 75(MedGEN)

  • myelodysplastic syndrome(DisGeNET, MedGEN)
    • loxP phage P1 loxP3loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAyeast FRT (flippase recombination target) site, phage P1 loxP site, mouse Tet2 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data