Strain Data Sheet

RBRC10850

Strain Information

Image
BRC No.RBRC10850
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Tet3<tm1.1Hko>
Former Common nameTET3
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN in 2011. M1 ((129 x C57BL/6)F1) ES cells were used. This strain was backcrossed to C57BL/6J.
Strain descriptionTet3 gene floxed mice. Gene targeting for the Tet3 allele was done by flanking exons 8 and 9 with Cre-loxP sequences. Conditional deletion of Tet3 (exons 8 and 9) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The TET3 protein is a dioxygenase that catalyzes the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and further oxidized cytosine (5fC and 5caC), and plays a key role in active DNA demethylation. Gdf9-Cre-driven Tet3 deletion in early oocytes resulted in a complete absence of Tet3 messenger RNA in Tet3mat-MII oocytes and Tet3 protein in zygotes (Amouroux et al., 2016). This results in a lack of 5hmC formation in the paternal pronucleus following fertilization in zygotes. This conditional allele would be useful to study the roles of Tet3 in DNA de-methylation by combining with tissue specific Cre deleters.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
Tet3
MGI:2446229		tet methylcytosine dioxygenase 36Tet3<tm1.1Hko> targeted mutation 1.1, Haruhiko Koseki
  • Beck-Fahrner syndrome(MedGEN)
    • loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP1 phage loxP sites, yeast FRT (flipase recombination target) site, mouse Tet3 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHaruhiko Koseki (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data