Strain Data Sheet
RBRC10852
Strain Information | |
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| Image | |
| BRC No. | RBRC10852 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Cst7<tm1Ike> |
| Former Common name | CysF STOPtetO knockin |
| H-2 Haplotype | |
| ES Cell line | RENKA [C57BL/6NCrlCrlj] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Kazuhiro Ikenaka at National Institute for Physiological Sciences in collaboration with Kenji Sakimura at Niigata University. RENKA ES cells derived from C57BL/6N were used to generate this strain. C57BL/6N background. Homozygous mice are viable and fertile. |
| Strain description | Knockin mice in which a STOP-tetO cassette was placed upstream of the translation initiation site of the Cst7 (Cystatin F) gene.Inserted DNA cassette contains loxP-FRT-Neo-poly A-FRT-tetO-loxP. |
| Colony maintenance | |
| References | The balance between cathepsin C and cystatin F controls remyelination in the brain of Plp1-overexpressing mouse, a chronic demyelinating disease model. Shimizu T, Wisessmith W, Li J, Abe M, Sakimura K, Chetsawang B, Sahara Y, Tohyama K, Tanaka K F, Ikenaka K Glia, 65(6):917-930 (2017). 28251676 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Cst7 MGI:1298217 | cystatin F (leukocystatin) | 2 | Cst7<tm1Ike> MGI:5910746 | targeted mutation 1, Kazuhiro Ikenaka | |||
| Frt | yeast FRT (flippase recombination target) site | 2 | Frt | ||||
| loxP | phage P1 loxP | 2 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 2 | mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage) | ||||
| SV40 polyA signal | 2 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | マウス PGK プロモーター, synthetic bacterial EM7 promoter (T7 phage), トランスポゾン Tn5由来 Neo 耐性遺伝子, tetracycline operator site 酵母 His3 5’ UTR, FRT SV40 polyA シグナル CMV 最小プロモーター バクテリオファージP1 loxP |
| Research application | Cre/loxP system FLP/frt system Tet system |
| Specific Term and Conditions | No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.) |
| Depositor | Nobuhiko Ohno (National Institute for Physiological Sciences) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |