Strain Data Sheet

RBRC10895

Strain Information

Image
BRC No.RBRC10895
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Gjc1<tm2.3Kni>
Former Common nameCx45 floxed; Cx45 flox; Connexin45 floxed; Connexin45 flox
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeposited by Kiyomasa Nishii, National Defense Medical College in 2019. CCE ES cell line derived from 129/SvEv was used. Crossing with a Cre strain removed a floxed neo selection cassette, but left the floxed entire coding exon of the Cx45 gene.
Strain descriptionFloxed Cx45 (Gjc1; gap junction protein, gamma 1). The entire coding exon was flanked with loxP sites followed by an nls-LacZ. This strain lacks the neo selection cassette by prior crossing with a Cre strain.
Colony maintenance
References
Mice lacking connexin45 conditionally in cardiac myocytes display embryonic lethality similar to that of germline knockout mice without endocardial cushion defect.
Nishii K, Kumai M, Egashira K, Miwa T, Hashizume K, Miyano Y, Shibata Y
Cell Commun. Adhes., 10:365-369 (2003). 14681043

Connexin45 contributes to global cardiovascular development by establishing myocardial impulse propagation.
Nishii K, Seki A, Kumai M, Morimoto S, Miwa T, Hagiwara N, Shibata Y, Kobayashi Y
Mech. Dev., 40:41-52 (2016). 26916723

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gjc1
MGI:95718		gap junction protein, gamma 111Gjc1<tm2.3Kni> targeted mutation 2.3, Kiyomasa Nishii
lacZ beta-galactosidase (E. coli)11
loxP phage P1 loxP11loxP
loxP phage P1 loxP11loxP
nls SV40 large T antigen nuclear localization signal (NLS)11nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAphage P1 loxP sites, SV40 large T antigen nuclear localization signal (NLS), E. coli beta-galactosidase (lacZ), SV40 polyA signal, mouse Gjc1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nishii K et al., Mech Dev. 2016 May;140:41-52.
DepositorKiyomasa Nishii (National Defense Medical College)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data