Strain Data Sheet

RBRC10908

Strain Information

Image
BRC No.RBRC10908
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-c/c Tg(Hdc-GFP)1Mpu
Former Common nameTg Hdc-GFP line1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takashi Moriguchi, Tohoku Medical and Pharmaceutical University in 2019. A BAC TG construct was injected to fertilized eggs derived from B6D2F1. Mice were backcrossed to a B6 albino strain for 4 generations.
Strain descriptionBAC transgenic mice harboring GFP reporter under the control of Hdc(histidine decarboxylase) gene. Albino strain.
Colony maintenance
References
Lipopolysaccharide-induced expansion of histidine decarboxylase-expressing Ly6G(+) myeloid cells identified by exploiting histidine decarboxylase BAC-GFP transgenic mice.
Takai J, Ohtsu H, Sato A, Uemura S, Fujimura T, Yamamoto M, Moriguchi T
Sci. Rep., 9(1):15603 (2019). 31666556

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tyr
MGI:98880		tyrosinase7Tyr<c-2J>
MGI:1855985		albino 2 Jackson
  • oculocutaneous albinism type 1A(MedGEN)

  • oculocutaneous albinism type 1B(MedGEN)

  • oculocutaneous albinism(DisGeNET)
    • Frt yeast FRT (flippase recombination target) siteUNFrt
    GFP Green Fluorescent Protein (Aequorea victoria)UN
    loxP phage P1 loxPUNloxP
    loxP phage P1 loxPUNloxP
    SV40 polyAUN

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAmouse histidine decarboxylase genomic DNA (BAC clone RP23-40N15), jellyfish GFP cDNA, SV40 polyA, Saccharomyces cerevisiae FRT site, E. coli chloramphenicol resistance gene, Phage P1 loxP sites derived from pBACe3.6 vector
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 9(1):15603 (2019).Depending on the content of the research, the DEPOSITOR may request collaboration. The RECIPIENT must contact the DEPOSITOR in the case of a application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTakashi Moriguchi (Tohoku Pharmaceutical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data