Strain Data Sheet

RBRC10920

Strain Information

Image
BRC No.RBRC10920
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1(mVenus/Cdkn1b<*>)Toki>
Former Common nameG0 marker mice
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped and deposited by Toshio Kitamura in 2019. ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic line.
Strain descriptionKnockin mouse strain at the ROSA26 locus in which mVenus-fused p27K can be overexpressed in a Cre-dependent manner.
Colony maintenance
References
Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium.
Fukushima T, Tanaka Y, Hamey F K, Chang C H, Oki T, Asada S, Hayashi Y, Fujino T, Yonezawa T, Takeda R, Kawabata K C, Fukuyama T, Umemoto T, Takubo K, Takizawa H, Goyama S, Ishihama Y, Honda H, Gottgens B, Kitamura T
Cell Rep., 29(12):4144-4158 (2019). 31851939

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cdkn1b
MGI:104565		cyclin dependent kinase inhibitor 1B6Cdkn1b
  • multiple endocrine neoplasia type 4(MedGEN)
    • Gt(ROSA)26Sor
    MGI:104735    		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(mVenus/Cdkn1b<*>)Toki> targeted mutation 1, Toshio Kitamura
    SA adenovirus Splicing acceptor6SA
    loxP phage P1 loxP6loxP
    loxP phage P1 loxP6loxP
    mVenus Yellow Fluorescent Protein (Aequorea victoria)6mVenus
    neo neomycin resistance gene (E. coli)6 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    SV40 polyA signal6

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAadenovirus Splicing acceptor, P1 phage LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistant gene, HSV tk polyA, Aequorea victoria mVenus cDNA, mouse p27K-(mutant p27) gene cDNA, SV40 virus PolyA signal, mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorToshio Kitamura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data