Strain Information | |
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Image | |
BRC No. | RBRC10926 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6.Cg-Hcn4<tm1.1(Luc/EGFP)Nna> |
Former Common name | HCN4 +/luc mice |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Makoto Takano, Kurume University in 2015. Bruce 4 ES cells were used. C57BL/6 congenic strain. |
Strain description | Reporter strain in which luciferase/EGFP fusion gene was inserted in frame of translation initiation codon of the Hcn4 gene. Hcn4 gene is disrupted. |
Colony maintenance | |
References | J. Physiol., 596(5):809-825 (2018). 29315578 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1.1(Luc/EGFP)Nna>targeted mutation 1, Noriyuki Nakashima Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)9EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP9loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lucluciferase9luc Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal9 |
Ordering Information | |
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Donor DNA | firefly Luciferase cDNA(U47298), jellyfish EGFP cDNA(U03453.1), Simian virus SV40 pA, P1 phage loxP site, mouse Hcn4 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 596(5):809-825 (2018).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Makoto Takano (Kurume University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |