Strain Data Sheet

RBRC10926

Strain Information

Image
BRC No.RBRC10926
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hcn4<tm1.1(Luc/EGFP)Nna>
Former Common nameHCN4 +/luc mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Makoto Takano, Kurume University in 2015. Bruce 4 ES cells were used. C57BL/6 congenic strain.
Strain descriptionReporter strain in which luciferase/EGFP fusion gene was inserted in frame of translation initiation codon of the Hcn4 gene. Hcn4 gene is disrupted.
Colony maintenance
References
HCN4 pacemaker channels attenuate the parasympathetic response and stabilize the spontaneous firing of the sinoatrial node.
Kozasa Y, Nakashima N, Ito M, Ishikawa T, Kimoto H, Ushijima K, Makita N, Takano M
J. Physiol., 596(5):809-825 (2018). 29315578

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)9EGFP
Hcn4
MGI:1298209		hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1.1(Luc/EGFP)Nna> targeted mutation 1, Noriyuki Nakashima
  • Brugada syndrome 8(MedGEN)

  • sick sinus syndrome 2, autosomal dominant(MedGEN)
    • loxP phage P1 loxP9loxP
    luc luciferase9luc
    SV40 polyA signal9

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAfirefly Luciferase cDNA(U47298), jellyfish EGFP cDNA(U03453.1), Simian virus SV40 pA, P1 phage loxP site, mouse Hcn4 genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 596(5):809-825 (2018).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorMakoto Takano (Kurume University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data