Strain Data Sheet
RBRC10928
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10928 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | C57BL/6J-Ucp1<em1(iRFP720)Utgr> |
| Former Common name | Ucp1-iRFP720 KI mice |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Aya Fukuda, University of Tsukuba in 2017. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. Homozygous mice are fertile. |
| Strain description | A reporter strain in which iRFP720 is placed around the translation initiation codon of the Ucp1 gene. |
| Colony maintenance | |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| GH | Growth hormone polyA (Bovine) | 8 | GH | ||||
| HBB | human beta-globin intron | 8 | HBB | ||||
| Ucp1 MGI:98894 | uncoupling protein 1 (mitochondrial, proton carrier) | 8 | Ucp1<em1(iRFP720)Utgr> | endonuclease-mediated mutation 1, Koji Hisataka | |||
| iRFP720 | Deinococcus radiodurans | 8 | iRFP720 | ||||
| human IgH intron | 8 | ||||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mus musculus Ucp1 genomic DNA, Homo sapiens IgH intron, Homo sapiens beta-globin intron, Mus musculus Kozak sequence, Deinococcus radiodurans iRFP720, Bos taurus bGH polyA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) |
| Depositor | Aya Fukuda (University of Tsukuba) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Mouse of the Month Feb 2021 |
BRC mice in Publications |
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