Strain Data Sheet
RBRC10931
Strain Information | |
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| Image | |
| BRC No. | RBRC10931 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.Cg-Tg(PDGFB-CLSTN1<*>CTF)Tg54Shat |
| Former Common name | Human Alcadein alpha/calcyntenin 1 CTF Tg mouse |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed and deposited by Norio Takei, Saori Hata, Toshiharu Suzuki, Hokkaido University in 2019. Transgenic DNA was injected to fetilized eggs derived from BDF1. C57BL/6J congenic strain by backcrossing for 15 generations. |
| Strain description | Transgenic mice expressing a c-terminal fragment of the human Alc alpha under the control of PDGF-beta promoter. |
| Colony maintenance | |
| References | Alcadein cleavages by amyloid beta-precursor protein (APP) alpha- and gamma-secretases generate small peptides, p3-Alcs, indicating Alzheimer disease-related gamma-secretase dysfunction. Hata S, Fujishige S, Araki Y, Kato N, Araseki M, Nishimura M, Hartmann D, Saftig P, Fahrenholz F, Taniguchi M, Urakami K, Akatsu H, Martins R N, Yamamoto K, Maeda M, Yamamoto T, Nakaya T, Gandy S, Suzuki T J. Biol. Chem., 284, 36024-36033 (2009). 19864413Cytoplasmic fragment of Alcadein alpha generated by regulated intramembrane proteolysis enhances amyloid beta-protein precursor (APP) transport into the late secretory pathway and facilitates APP cleavage. Takei N, Sobu Y, Kimura A, Urano S, Piao Y, Araki Y, Taru H, Yamamoto T, Hata S, Nakaya T, Suzuki T J. Biol. Chem., 290, 987-995 (2015). 25406318 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| CLSTN1 | calsyntenin 1 (human) | UN | CLSTN1 | human PDGF-beta promoter | |||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | human PDGF-beta promoter, human Alc alpha cDNA, SV40 polyA sequence |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 284, 36024-36033 (2009). J. Biol. Chem., 290, 987-995 (2015). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Original researcher Drs. Toshiharu Suzuki & Saori Hata is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-proflt research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval form Drs. Toshiharu Suzuki (tsuzuki@pharm.hokudai.ac.jp) and Saori Hata (shata@pharm.hokudai.ac.jp) using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-proit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with Drs. Toshiharu Suzuki & Saori Hata and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact Drs. Toshiharu Suzuki & Saori Hata in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Saori Hata (Hokkaido University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |