Strain Data Sheet

RBRC10946

Strain Information

Image
BRC No.RBRC10946
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Alpl<tm1(cre)Nagy>/Evo
Former Common nameTNAP-Cre
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeposited by Kazyo Kinoshita, Shiga Medical Center Research Institute in 2019. This strain was backcrossed to C57BL/6J for 20 generations.
Strain descriptionCre knockin mice at the TNAP (Alpl) locus.
Colony maintenance
References
Targeted insertion of Cre recombinase into the TNAP gene: excision in primordial germ cells.
Lomeli H, Ramos-Mejia V, Gertsenstein M, Lobe C G, Nagy A
Genesis, 26(2):116-7 (2000). 10686602

Stepwise generation of AID knock-in and conditional knockout mice from a single gene-targeting event.
Kinoshita K, Uemura M, Shimizu T, Kinoshita S, Marusawa H
Int. Immunol., 33(7):387-398 (2021). 33903914

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Alpl
MGI:87983		alkaline phosphatase, liver/bone/kidney4Alpl<tm1(cre)Nagy> targeted mutation 1,
  • obsolete adult hypophosphatasia(DisGeNET)

  • obsolete childhood hypophosphatasia(DisGeNET)

  • obsolete infantile hypophosphatasia(DisGeNET)
    • IRES internal ribosomal entry site (EMCV)4
    cre Phage P1 Cre recombinase4cre
    neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAencephalomyocarditis virus IRES, Bacteriophage P1 Cre gene, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, unknown Pgk1 PolyA signal
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 26(2):116-7 (2000). Int Immunol., 33(7):387-398 (2021). For for-profit institutions - please contact Sinai Health System for a license : Michael Hanna, Sr. Busmers Officer hanna@lunenfeld.ca
    DepositorKazuo Kinoshita (Shiga Medical Center Research Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data