Strain Data Sheet
RBRC10958
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10958 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | C57BL/6-Gcg<em1(cre)Utr> |
| Former Common name | Gcg-Cre |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Satoru Takahashi, University of Tsukuba in 2020. C57BL/6J genetic background. |
| Strain description | Cre recombinase was placed at 3’ termini of protein coding of the Gcg gene via 2A peptide. |
| Colony maintenance | |
| References | Uncovering the role of MAFB in glucagon production and secretion in pancreatic alpha-cells using a new alpha-cell-specific Mafb conditional knockout mouse model. Chang Y H, Katoh M C, Abdellatif A M, Xiafukaiti G, Elzeftawy A, Ojima M, Mizuno S, Kuno A, Takahashi S Exp. Anim., 69(2):178-188 (2020). 31787710 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/06/09Room:3-3Rack:ASentinel mouse program 2025/03/10Room:3-3Rack:ASentinel mouse program 2024/12/09Room:3-3Rack:ASentinel mouse program 2024/09/09Room:3-3Rack:ASentinel mouse program 2024/06/10Room:3-3Rack:ASentinel mouse program 2024/03/11Room:3-3Rack:ASentinel mouse program 2023/12/11Room:3-3Rack:ASentinel mouse program 2023/09/11Room:3-3Rack:ASentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Gcg MGI:95674 | glucagon | 2 | Gcg<em1(cre)Utr> | endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center | |||
| HBB2 | rabbit bata-globin polyA | 2 | HBB2 | ||||
| P2A | porcine teschovirus-1 2A peptide | 2 | P2A | ||||
| cre | Phage P1 Cre recombinase | 2 | cre | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | 2 | nls | ||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | P2A (porcine teschovirus-1 2A peptide), SV40 nls, P1 phage Cre recombinase, rabbit beta-globin polyA |
| Research application | Cre/loxP system Cre driver |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 69(2):178-188 (2020). |
| Depositor | Satoru Takahashi (University of Tsukuba) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |