Strain Data Sheet

RBRC10966

Strain Information

Image
BRC No.RBRC10966
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Gt(ROSA)26Sor<em1(CAG-venus,-mCherry,-mCerulean)Okn>/26
Former Common nameROSA26<MultiFPs Delta Puro>
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionFluorescent reporter mice for three difference recombinases. Expression of Venus, mCherry and mCerulean under the control of CAG promoter at the ROSA26 locus can be induced by Cre, Dre and phiC31o, respectively.
Colony maintenance
References
Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers.
Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H
Development, 146(21):dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2024/09/09Room:3-3Rack:ASentinel mouse program
2024/06/10Room:3-3Rack:ASentinel mouse program
2024/03/11Room:3-3Rack:ASentinel mouse program
2023/12/11Room:3-3Rack:ASentinel mouse program
2023/09/11Room:3-3Rack:ASentinel mouse program
2023/06/13Room:3-3Rack:ASentinel mouse program
2023/03/13Room:3-3Rack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site6Frt
GH Growth hormone polyA (Bovine)6GH
Gt(ROSA)26Sor
MGI:104735		gene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<em1(CAG-venus,-mCherry,-mCerulean)Okn> endonuclease mediated mutation 1, Hideyuki Okano
Venus Yellow Fluorescent Protein (Aequorea victoria)6Venus
attB attachment site6attB
attP attachment site6attP
loxP phage P1 loxP6loxP
loxP phage P1 loxP6loxP
mCerulean Cyan Fluorescent protein (Aequorea victoria)6mCerulean
mCherry Red Fluorescent Protein (Discosoma sp.)6mCherry
puro puromycin acyltransferase (Streptomyces alboniger)6puro CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
rox Bacteriophage D6 rox site6rox
rox Bacteriophage D6 rox site6rox
SV40 polyA signal6

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), bacteriophage P1 loxP sites, Streptomyces lividans attB site, P1-like transducing phage D6 rox sites, Streptomyces alboniger Puromycin resistance gene, jellyfish GFP variant (Venus) cDNA, Entacmaea quadricolor RFP variant (mCherry) cDNA, SV40 polyA, Streptomyces lividans attP site, jellyfish GFP variant (mCerulean)cDNA, bovine growth hormone polyA, Saccharomyces cerevisia FRT site, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 146(21). pii: dev174938 (2019). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (TBUSAMTA_Nonprofit_BROAD)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-
Mouse of the Month Apr 2022

BRC mice in Publications

No Data