Strain Data Sheet

RBRC10967

Strain Information

Image
BRC No.RBRC10967
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Sox1<em1(cre)Okn> T<em1(dre)Okn> Sox17<em1(phiC31<*>)Okn>/2-3
Former Common nameTriple lineagelabelling mice (Sox12A-Cre Delta Puro, brachyury2A-Dre Delta Puro, Sox172A-phiC31o Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionTriple knockin for three different recombinases: Sox1-Cre, T-Dre and Sox17-PhiC31<*>.All recombinases were knockined at the C-terminus of protein coding sequence of the respective gene allele.
Colony maintenance
References
Developmental analyses of mouse embryos and adults using a non-overlapping tracing system for all three germ layers.
Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, Okano H
Development, 146(21):dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2023/08/28Room:4-BRack:BSentinel mouse program
2023/05/29Room:4-BRack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
F5 yeast FRT (flippase recombination target) site (synthetic) variant1F5
P2A porcine teschovirus-1 2A peptide1P2A
PhiC31o PhiC31 recombinase1PhiC31o rat tyrosine hydroxylase (TH) promoter
Sox17
MGI:107543		SRY (sex determining region Y)-box 171Sox17<em1(phiC31<*>)Okn> endonuclease-induced mutation 1, Hideyuki Okano
  • vesicoureteral reflux 3(MedGEN)
    • nls SV40 large T antigen nuclear localization signal (NLS)1nls
    Dre Bacteriophage D6 recombinase17Dre
    F3 yeast FRT (flippase recombination target) site (synthetic) variant17F3
    P2A porcine teschovirus-1 2A peptide17P2A
    T
    MGI:98472    		brachyury, T-box transcription factor T17T<em1(dre)Okn> endonuclease-induced mutation 1, Hideyuki Okano
  • neural tube defect(MedGEN)

  • sacral agenesis-abnormal ossification of the vertebral bodies-persistent notochordal canal syndrome(MedGEN)
    • nls SV40 large T antigen nuclear localization signal (NLS)17nls
    Frt yeast FRT (flippase recombination target) site8Frt
    P2A porcine teschovirus-1 2A peptide8P2A
    Sox1
    MGI:98357    		SRY (sex determining region Y)-box 18Sox1<em1(cre)Okn> endonuclease-induced mutation 1, Hideyuki Okano
    cre Phage P1 Cre recombinase8cre
    nls SV40 large T antigen nuclear localization signal (NLS)8nls

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), SV40 virus NLS, bacteriophage P1 Cre, Saccharomyces cerevisia FRT site, mouse Sox1 genomic DNA, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, P1-like transducing phage D6 Dre, SV40 virus NLS, Saccharomyces cerevisia FRT site(F3), mouse T/brachyury genomic DNA, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, Streptomyces lividans phiC31o, Saccharomyces cerevisia FRT site(F5), mouse Sox17 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 146(21). pii: dev174938 (2019).
    DepositorHideyuki Okano (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-
    Mouse of the Month Apr 2022

    BRC mice in Publications

    No Data